Table 1. Absorption Maxima, Emission Maxima, Stokes Shift Values, and Quantum Yield of Thioflavin-T in Six Glycerol–Monohydroxy Alcohol Solvent Mixtures along with the Known Values of Bulk Viscosity of the Mixtures.
| X1 | X2 | absorption maxima in cm–1 (in nm) | emission maxima in cm–1 (in nm) | Stokes shift in cm–1 | quantum yield of ThTa | viscosity (cP)b,c |
|---|---|---|---|---|---|---|
| Glycerol (1) + Methanol (2) | ||||||
| 0.000 | 1.000 | 24073.18 (415.4) | 21186.44 (472.0) | 2886.74 | 0.0005 | 0.6 |
| 0.058 | 0.942 | 24026.91 (416.2) | 21164.02 (472.5) | 2862.89 | 0.0012 | 1.8 |
| 0.122 | 0.878 | 23969.32 (417.2) | 21097.05 (474.0) | 2872.27 | 0.0018 | 4.8 |
| 0.192 | 0.808 | 23934.9 (417.8) | 21008.40 (476.0) | 2926.50 | 0.0022 | 7.7 |
| 0.270 | 0.730 | 23877.75 (418.8) | 20898.64 (478.5) | 2979.10 | 0.0030 | 13 |
| 0.357 | 0.643 | 23843.59 (419.4) | 20768.43 (481.5) | 3075.15 | 0.0041 | 28 |
| 0.454 | 0.546 | 23809.52 (420.0) | 20682.52 (483.5) | 3127.0 | 0.0063 | 58 |
| 0.564 | 0.436 | 23775.56 (420.6) | 20597.32 (485.5) | 3178.24 | 0.0082 | 130 |
| 0.689 | 0.311 | 23741.69 (421.2) | 20554.98 (486.5) | 3186.71 | 0.0128 | 250 |
| 0.833 | 0.167 | 23707.92 (421.8) | 20533.88 (487.0) | 3174.04 | 0.0200 | 630 |
| 1.000 | 0.000 | 23707.92 (421.8) | 20576.13 (486.0) | 3131.79 | 0.0963 | 950 |
| Glycerol (1) + Ethanol (2) | ||||||
| 0.000 | 1.000 | 23900.57 (418.4) | 21276.6 (470.0) | 2623.98 | 0.0013 | 1.23 |
| 0.082 | 0.918 | 23866.35 (419.0) | 21164.02 (472.5) | 2702.33 | 0.0021 | 2.81 |
| 0.167 | 0.833 | 23820.87 (419.8) | 21008.4 (476.0) | 2812.46 | 0.0026 | 6.28 |
| 0.255 | 0.745 | 23820.87 (419.8) | 20876.83 (479.0) | 2944.04 | 0.0034 | 13.70 |
| 0.348 | 0.652 | 23798.19 (420.2) | 20811.65 (480.5) | 2986.54 | 0.0048 | 29.01 |
| 0.444 | 0.556 | 23775.56 (420.6) | 20746.89 (482.0) | 3028.67 | 0.0063 | 59.24 |
| 0.545 | 0.455 | 23764.26 (420.8) | 20639.83 (484.5) | 3124.42 | 0.0089 | 115.76 |
| 0.651 | 0.349 | 23741.69 (421.2) | 20597.32 (485.5) | 3144.37 | 0.0129 | 214.68 |
| 0.762 | 0.238 | 23730.42 (421.4) | 20576.13 (486.0) | 3154.29 | 0.0221 | 374.08 |
| 0.878 | 0.122 | 23696.68 (422.0) | 20576.13 (486.0) | 3120.55 | 0.0373 | 605.47 |
| 1.000 | 0.000 | 23707.92 (421.8) | 20576.13 (486.0) | 3131.79 | 0.0963 | 950.0 |
| Glycerol (1) + n-Propanol (2) | ||||||
| 0.000 | 1.000 | 23900.57 (418.4) | 20898.64 (478.5) | 3001.93 | 0.0024 | |
| 0.103 | 0.897 | 23866.35 (419.0) | 20811.65 (480.5) | 3054.69 | 0.0029 | |
| 0.205 | 0.795 | 23832.22 (419.6) | 20790.02 (481.0) | 3042.20 | 0.0038 | |
| 0.306 | 0.694 | 23809.52 (420.0) | 20746.89 (482.0) | 3062.64 | 0.0057 | |
| 0.407 | 0.593 | 23798.19 (420.2) | 20725.39 (482.5) | 3072.80 | 0.0070 | |
| 0.507 | 0.493 | 23764.26 (420.8) | 20661.16 (484.0) | 3103.10 | 0.0108 | |
| 0.607 | 0.393 | 23730.42 (421.4) | 20639.83 (484.5) | 3090.59 | 0.0148 | |
| 0.706 | 0.294 | 23707.92 (421.8) | 20618.56 (485.0) | 3089.36 | 0.0203 | |
| 0.805 | 0.195 | 23696.68 (422.0) | 20597.32 (485.5) | 3099.36 | 0.0304 | |
| 0.903 | 0.097 | 23674.24 (422.4) | 20554.98 (486.5) | 3119.26 | 0.0440 | |
| 1.000 | 0.000 | 23707.92 (421.8) | 20576.13 (486.0) | 3131.79 | 0.0963 | |
| Glycerol (1) + iso-Propanol (2) | ||||||
| 0.000 | 1.000 | 23957.83 (417.4) | 20898.64 (478.5) | 3059.19 | 0.0027 | |
| 0.105 | 0.895 | 23889.15 (418.6) | 20833.33 (480.0) | 3055.82 | 0.0038 | |
| 0.208 | 0.792 | 23866.35 (419.0) | 20833.33 (480.0) | 3033.02 | 0.0050 | |
| 0.311 | 0.689 | 23832.22 (419.6) | 20790.02 (481.0) | 3042.20 | 0.0073 | |
| 0.413 | 0.587 | 23798.19 (420.2) | 20725.39 (482.5) | 3072.80 | 0.0106 | |
| 0.513 | 0.487 | 23764.26 (420.8) | 20703.93 (483.0) | 3060.32 | 0.0138 | |
| 0.612 | 0.388 | 23752.97 (421.0) | 20682.52 (483.5) | 3070.45 | 0.0209 | |
| 0.711 | 0.289 | 23719.17 (421.6) | 20618.56 (485.0) | 3100.61 | 0.0286 | |
| 0.808 | 0.192 | 23696.68 (422.0) | 20597.32 (485.5) | 3099.36 | 0.0415 | |
| 0.905 | 0.095 | 23685.46 (422.2) | 20554.98 (486.5) | 3130.47 | 0.0540 | |
| 1.000 | 0.000 | 23696.68 (422.0) | 20576.13 (486.0) | 3120.55 | 0.0963 | |
| Glycerol (1) + n-Butanol (2) | ||||||
| 0.000 | 1.000 | 23889.15 (418.6) | 20876.83 (479.0) | 3012.33 | 0.0043 | 2.57 |
| 0.122 | 0.878 | 23854.96 (419.2) | 20855.06 (479.5) | 2999.90 | 0.0058 | 4.78 |
| 0.239 | 0.761 | 23820.87 (419.8) | 20790.02 (481.0) | 3030.85 | 0.0081 | 8.90 |
| 0.350 | 0.650 | 23786.87 (420.4) | 20746.89 (482.0) | 3039.98 | 0.0110 | 16.52 |
| 0.455 | 0.545 | 23775.56 (420.6) | 20703.93 (483.0) | 3071.62 | 0.0141 | 30.56 |
| 0.556 | 0.444 | 23741.69 (421.2) | 20682.52 (483.5) | 3059.17 | 0.0197 | 56.24 |
| 0.653 | 0.347 | 23719.17 (421.6) | 20682.52 (483.5) | 3036.64 | 0.0260 | 102.83 |
| 0.745 | 0.255 | 23707.92 (421.8) | 20661.16 (484.0) | 3046.76 | 0.0334 | 186.66 |
| 0.834 | 0.166 | 23696.68 (422.0) | 20639.83 (484.5) | 3056.85 | 0.0471 | 336.12 |
| 0.919 | 0.081 | 23696.68 (422.0) | 20618.56 (485.0) | 3078.13 | 0.0637 | 600.13 |
| 1.000 | 0.000 | 23696.68 (422.0) | 20576.13 (486.0) | 3120.55 | 0.0963 | 950.0 |
| Glycerol (1) + tert-Butanol (2) | ||||||
| 0.000 | 1.000 | 23889.15 (418.6) | 20920.5 (478.0) | 2968.65 | 0.0050 | 3.15 |
| 0.126 | 0.874 | 23866.35 (419.0) | 20855.06 (479.5) | 3011.29 | 0.0077 | 8.22 |
| 0.246 | 0.754 | 23854.96 (419.2) | 20811.65 (480.5) | 3043.31 | 0.0122 | 18.72 |
| 0.358 | 0.642 | 23820.87 (419.8) | 20768.43 (481.5) | 3052.44 | 0.0171 | 37.85 |
| 0.465 | 0.535 | 23798.19 (420.2) | 20725.39 (482.5) | 3072.80 | 0.0240 | 69.04 |
| 0.566 | 0.434 | 23741.69 (421.2) | 20703.93 (483.0) | 3037.76 | 0.0292 | 115.12 |
| 0.661 | 0.339 | 23741.69 (421.2) | 20682.52 (483.5) | 3059.17 | 0.0358 | 177.45 |
| 0.752 | 0.248 | 23707.92 (421.8) | 20639.83 (484.5) | 3068.08 | 0.0429 | 255.31 |
| 0.839 | 0.161 | 23685.46 (422.2) | 20597.32 (485.5) | 3088.13 | 0.0479 | 345.77 |
| 0.921 | 0.079 | 23674.24 (422.4) | 20597.32 (485.5) | 3076.92 | 0.0520 | 444.00 |
| 1.000 | 0.000 | 23696.68 (422.0) | 20576.13 (486.0) | 3120.55 | 0.0780 | 544.03 |