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. 2023 Sep 21;56(19):2653–2663. doi: 10.1021/acs.accounts.3c00370

Figure 3.

Figure 3

Exchange dynamics of Zn(II)- and Ag(I)-Aβ complexes characterized by NMR relaxation dispersion experiments. (a, b) Distinct residues exhibit significantly high amplitude 15N CPMG relaxation dispersion profiles for Zn(II)-Aβ40 (blue) and Ag(I)-Aβ40 (green) complexes, where the absolute chemical shift changes are plotted, obtained from a global fit analysis. The temperature dependence of the relaxation dispersion profile for two selected residues, E3 and V12, is displayed, where circles correspond to 278 (red), 281 (yellow), 284 (green), 287 (blue), and 290 K (violet) in the presence of metal ions. Black squares reflect the dynamics without any metal ions added. (c, d) The population of the NMR invisible state and the exchange rate between the two states exhibit very similar temperature dependences for Zn(II) and Ag(I). (e) The binding model is visualized where in the NMR invisible state the N-terminus encapsulates the metal ion, forming a more compact “folded” complex. Data were replotted from refs (2) and (3).