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. 2023 May 26;77(7):987–990. doi: 10.1093/cid/ciad320

Figure 1.

Figure 1.

RNAscope 2.5 HD Assay-RED on FFPE skin of patients with parapsoriasis, counterstained with hematoxylin. A, B Sample AS11 hybridized with (A) human PPIB mRNA probe and (B) bacterial DapB probe, as positive and negative technical controls. C, D CuV VP probe on (C) CuV PCR-negative sample AS8 and (D) the CuV PCR-positive sample AS11. Representative positive probe signals (red punctuated dots) are indicated by black arrows and as a zoomed-in box at the bottom-right corner of panel A and four zoomed-in boxes at the right side of panel D. Scale bars, 20 μm (A–C) and 100 μm (D). As a further probe control, CuV RISH on insect cells with and without CuV VP-mRNA expression is shown in Supplementary Figure 3. Abbreviations: CuV, cutavirus; FFPE, formalin-fixed paraffin-embedded; PCR, polymerase chain reaction; RISH, RNAscope in situ hybridization.