KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | identifier |
|---|---|---|
|
| ||
| Antibodies | ||
|
| ||
| Rabbit polyclonal anti-eIF4E | Bethyl Laboratories | Cat# A301-154A, RRID:AB_2097708 |
| Rabbit monoclonal anti-eIF4G | Cell Signaling Technology | Cat# 2469, RRID:AB_2096028 |
| Mouse monoclonal anti-FMRP | BioLegend | Cat# 834701, RRID:AB_2564993 |
| Mouse monoclonal anti-Puromycin | Millipore | Cat# MABE343, RRID:AB_2566826 |
| Rabbit monoclonal anti-GAPDH | Cell Signaling Technology | Cat# 2118, RRID:AB_561053 |
| Rabbit poiycionai anti-RFP | Rockland | Cat# 600-401-379, RRID:AB_2209751 |
| Chicken poiycionai anti-GFP | Abcam | Cat# ab13970, RRID:AB_300798 |
| Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11039, RRID:AB_2534096 |
| Goat anti-Chicken secondary | ||
| Alexa Fluor 568 | Thermo Fisher Scientific | Cat# A-11011, RRID:AB_143157 |
| Goat anti-Rabbit secondary | ||
| TRAP anti-GFP 19C8 | N. Heintz Laboratory | RRID:AB_2716737 |
| TRAP anti-GFP 19F7 | Memorial Sloan Kettering Monoclonal Antibody facility/N. Heintz Laboratory | RRID:AB_2716736 |
| Anti-Mouse IgG (H + L) Antibody, HRP | Promega | Cat# W4021, RRID:AB_430834 |
| Conjugated | ||
|
| ||
| Bacterial and virus strains | ||
|
| ||
| AAV1 -Syn-GCaMP6f-WPRE-SV40 | Penn Vector Core | RRID:Addgene_100837 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| 4EGI-1 | Merk Sigma-Aldrich | Cat# 324517 |
| VU0152100 | Sigma-Aldrich | Cat# V5015 |
| γ-Aminophenyl-m7GTP (C10-spacer)-Agarose | Jena Bioscience | Cat# AC155 |
| Puromycin dihydrochloride | Sigma-Aldrich | Cat# P8833 |
| Halt™ Protease and Phosphatase Inhibitor Cocktail | ThermoFisher | Cat# 78441 |
| Azidohomoalanine (AHA) | Click Chemistry Tools | Cat# 1066-100 |
| Dapi-Fluoromount-G™ | Electron Microscopy Sciences | Cat# 17984-24 |
| Biotinylated protein L, recombinant, purified | Fisher Scientific | Cat# PI-29997 |
| Pierce™ Streptavidin Magnetic Beads | ThermoFisher | Cat# 88816 |
| Alexa Fluor™ 488 Alkyne | ThermoFisher | Cat# A10267 |
| Triethylammonium Salt | ||
| Alexa Fluor™ 647 Alkyne | ThermoFisher | Cat# A10278 |
| Triethylammonium Salt | ||
| Anisomycin | Tocris | Cat# 1290 |
|
| ||
| Critical commercial assays | ||
|
| ||
| ECL Prime Western Blotting Detection Reagent | GE Healthcare Amersham | Cat# 12316992 |
| Pierce™ BCA Protein Assay Kit | ThermoFisher | Cat# 23225 |
| Absolutely RNA Nanoprep Kit | Agilent | Cat# 400753 |
| Click-iT™ Cell Reaction Buffer Kit | ThermoFisher | Cat# C10269 |
|
| ||
| Deposited data | ||
|
| ||
| Raw RNA-Seq sequencing reads | This study | NCBI GEO: GSE165872 |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| Mouse: Fmr1 KO | Jackson Laboratory | RRID:IMSR_JAX:003025 |
| Mouse: Drd2-EGFP | GENSAT | RRID:MMRRC_000230-UNC |
| Mouse: Drd1a-tdTomato | Jackson Laboratory | RRID:IMSR_JAX:016204 |
| Mouse: Drd1a-EGFP/Rpl10a | Jackson Laboratory | RRID:IMSR JAX:030254 |
| Mouse: Fmr1f/f | Jackson Laboratory | RRID:IMSR_JAX:035184 |
| Mouse: Drd1-Cre | GENSAT | MMRRC_034258-UCD |
|
| ||
| Software and algorithms | ||
|
| ||
| ImageJ | NIH | N/A |
| GraphPad Prism version 9 | GraphPad Software | N/A |
| Activity Monitor 7 Software | Med Associated Inc | N/A |
| EthoVision XT 13 | Noldus | N/A |
| Huygens Professional | Scientific Volume Imaging | N/A |
| MATLAB | MathWorks | N/A |
| MATLAB custom-code | N. Tritsch Laboratory | https://github.com/TritschLab/TLab-2P-analysis |
| pClamp 10 | Molecular Devices | N/A |