Figure 4:
Processing and quantification of inflammation via detection of CD68 deposition by CellProfiler. A) Representative images of gastrocnemius muscle cross-sections from 4–6-month-old B10 and mdx mice immunolabeled with laminin α1 (red), and CD68 (green). Scale bar = 100μm. B) Boxplot illustrating the quantification of the sample CD68 dataset images showing the percent area of CD68 deposition in each gastrocnemius muscle of WT and mdx mice (n = 5 mice/group). **p< 0.01. C) Overview of the image analysis workflow. D) A sample mdx image was fed into the CD68 quantification pipeline which first converts the color image into separate grayscale images representing each color channel. The green channel grayscale image was used for further processing. E) Outlines of the identified CD68. F) The identified objects (CD68) were then converted into a binary black and white image for easy quantification of CD68 deposition (% area) within the muscle cross-section.