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. 2023 Jun 19;63(2):90. doi: 10.3892/ijo.2023.5538

Figure 5.

Figure 5

O-GlcNAcylation regulates YAP stability and phosphorylation by inhibiting the YAP/LATS1/2 interaction. (A and B) Western blot analysis of YAP and p-YAP expression levels in the Con-sh and OGT-sh groups of (A) AN3CA and (B) HEC-1-B cells. (C) mRNA expression levels of YAP in the Con-sh and OGT-sh groups of endometrial cancer cells. (D) CHX chase experiments were used to measure YAP expression at different time points following treatment of the cells with CHX (50 µM). (E) Co-immunoprecipitation assay revealed that O-GlcNAcylation affected the YAP-LATS1/2 interaction. Data represent the mean ± SD (n=3). **P<0.01. ns, not significant; YAP, Yes-associated protein; OGT, O-GlcNAc transferase; CHX, cycloheximide.