Skip to main content
. 2023 Jun 19;63(2):90. doi: 10.3892/ijo.2023.5538

Figure 6.

Figure 6

YAP O-GlcNAcylation promotes its activation and nuclear translocation. (A) Nuclear-cytoplasmic separation assays revealed the cellular sub-localization of YAP in the different groups. (B and C) Immunofluorescence staining revealed the cellular sub-localization of YAP in the different groups. Scale bars, 10 µm. (D and E) mRNA expression of ANKRD1, CTGF, CYR61 and GLUT3 was detected in endometrial cancer using reverse transcription-quantitative PCR in EC cells. (F) Western blot analysis was used to measure CTGF expression in endometrial cancer cells. Data represent the mean ± SD (n=3). *P<0.05 and **P<0.01. ns, not significant; YAP, Yes-associated protein; OGT, O-GlcNAc transferase; H3, Histone-H3; ANKRD1, ankyrin repeat domain 1; CTGF, connective tissue growth factor; CYR61, cysteine-rich angiogenic inducer 61; GLUT3, glucose transporter 3.