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. 2023 Jul 10;63(2):98. doi: 10.3892/ijo.2023.5546

Figure 5.

Figure 5

circRNA ribosomal protein S19 upregulates HK2 protein via USP7-mediated deubiquitination. (A) Co-IP analysis of the interaction between USP7 and HK2 in AGS cells. (B) Immunofluorescence assay detected the co-localization of USP7 and HK2 in AGS cells. WB of (C) HK2 protein expression in AGS cells and (D) degradation rate of HK2 protein in CHX-treated AGS cells following USP7 ablation and (E) HK2 protein expression following USP7 overexpression and HBX19818 treatment in AGS cells. (F) IP showed HK2 ubiquitination in MG132-treated AGS cells following USP7 depletion. *P<0.05, **P<0.01, ***P<0.001 vs. control group. HK2, hexokinase 2; USP7, ubiquitin-specific processing protease 7; IP, immunoprecipitation; CHX, cycloheximide; HBX19818, a specific inhibitor of USP7; si, small interfering; NC, negative control; WB, western blotting; Ub, ubiquitin.