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. 2023 May 30;63(1):81. doi: 10.3892/ijo.2023.5529

Figure 4.

Figure 4

LINC02038 exerts its function by inhibiting miR-552-5p in CRC cells. (A) miR-552-5p expression was determined using RT-qPCR after transfection with miR-552-5p mimic, LINC02038 overexpression + miR-552-5p mimic or the control. (B) CCK-8 and (C) colony formation assays were used to assess the proliferation of transfected cells. (D and E) Flow cytometry was used to identify the cell cycle distribution and apoptosis of SW620 and LoVo cells transfected with miR-552-5p mimic, LINC02038 overexpression + miR-552-5p mimic or the control. LINC02038 overexpression resulted in G1 phase cell cycle arrest and increased rates of apoptosis and partially reversed the effects of miR-552-5p mimic transfection in CRC cells. (F) Migration and (G) invasion rates were determined using Transwell assays in CRC cells co-transfected with LINC02038 overexpression vector and miR-552-5p mimics as well as the corresponding controls. *P<0.05, **P<0.01 vs. NC mimic; miR, microRNA; CRC, colorectal cancer; RT-qPCR, reverse transcription quantitative PCR; NC, negative control.