Fig. 2.

The effect of single nucleotide polymorphism rs4131564 on T-cell differentiation, exhaustion, senescence and proliferation in people with HIV.

Flowcytometry analysis of PBMC from PWH carrying the major or minor allele of rs4131564: CD4⁺ and CD8⁺ T-cell differentiation as determined by CD45RA and CD27 expression (a and b); exhaustion as determined by PD-1 expression and senescence as determined by CD57⁺ expression or CD27^–/CD28^– expression (c and d); percentage CD4⁺ and CD8⁺ T-cell proliferation as determined by the loss of CellTrace Violet, upon 6 days of culture with anti-CD3 and anti-CD28 antibody or HIV-1 gag peptide pool stimulation (e and f). PBMC, peripheral blood mononuclear cells; PWH, people with HIV.