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. Author manuscript; available in PMC: 2024 Jul 29.
Published in final edited form as: Lancet. 2023 Jun 29;402(10399):397–410. doi: 10.1016/S0140-6736(23)00775-4

Figure 5: PCMV/PRV Investigations.

Figure 5:

i: shows the PCMV/PRV DNA level detected in the pig tissue’s initial screening from euthanasia and patient PBMCs from POD 45, as determined by qPCR normalized to one cellular load of porcine DNA, ii: confirmation of the initial PCMV/PRV investigation by conventional PCR for PCMV/PRV DNA, following electrophoresis on a 1.5% agarose gel. Lane 1: patient PBMCs, lane 2: pig spleen, lane 3: positive control, lane 4: negative control, lane 5: empty, lane 6: 100bp ladder, iii: PCMV/PRV DNA level detected in the xenograft compartments and recipient organs at autopsy; as determined by qPCR, PCMV/PRV DNA levels are normalized to one cellular load of porcine DNA, and iv: PCMV/PRV RNA transcripts detected within the right ventricle of the xenograft at autopsy, as determined by qRT-PCR. Transcript copies are normalized to 100ng of total RNA.