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The D7 embryo morphologies (A) and blastocyst rate (B) at 0 h, AGED-24 h, AGED+NMN-24 h groups. Scale bars represented 100 μm. Expression of pluripotent genes NANOG (C) OCT4 (D) and SOX2 (E) at 0 h, AGED-24 h, AGED+NMN-24 h groups. *p < 0.05, **p < 0.01 indicated significant differences. (F) and (G) Statistics of the number of blastocysts at 0 h, AGED-24 h, AGED+NMN-24 h groups. Scale bars represented 50 μm.
