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Restriction map and subcloning of the YOR1 locus. The restriction map of an 8.5-kb genomic insert of pWL7 is shown at the top. The thick arrow indicates the location and direction of an ORF. DNAs subcloned on the pWH5 vector were examined for their ability to confer AbA resistance on wild-type cells. B, BamHI; Bs, BstXI; E, EcoRI; H, HindIII; N, NheI; P, PstI; T, Tth111I. +, resistance conferred; −, resistance not conferred.
