FIG. 4.
Northern blot analysis of the YOR1 gene in aur3R mutants. (A) Total RNA from wild-type DKD-5D cells (lanes 1, 3, and 5) or aur3R mutant AL22-4A cells (lanes 2, 4, and 6) was studied by Northern hybridization. The probes used were a 1.2-kb HindIII-PstI fragment of YOR1 (lanes 1 and 2), a 1.1-kb PDR5 fragment (lanes 3 and 4), and a 1.5-kb actin DNA fragment (lanes 5 and 6). (B) Genomic DNAs from DKD-5D cells (lane 7) and AL22-4A cells (lane 8) were digested with HindIII and studied by Southern hybridization with the 1.0-kb NheI-BstXI fragment of YOR1 as the probe (see Fig. 1).