Fig 3. Inhibiting choline metabolism drives pronounced changes in gene transcription in naïve and IL-4-polarized macrophages.

A) Schematic of bulk RNA-seq sample preparation. Heatmap of z-scores for select genes of interest. n = 3. B) Volcano plots of comparisons between Veh and HC3 or RSM in M[0] or M[IL-4]. Red dots represent genes with false discovery rate (FDR) < 0.05 and log₂ fold change (FC) < -1 or > 1. Select genes of interest are annotated. C-D) The top significantly up- or down-regulated genes shared in all comparisons in B) were analyzed by Enrichr-KG to identify common pathways. Schematics were created using BioRender.