TABLE 1.
Comparison of silicon oil and centrifugation methods
| Time (min) | Silicon oil separation
method
|
Centrifugation separation method
|
||||
|---|---|---|---|---|---|---|
| Level (ng/mg) of binding at 0°C | Level (ng/mg) of accumulation at 37°C | Level (ng/mg) of intracellular accumulation | Level (ng/mg) of binding at 0°C | Level (ng/mg) of accumulation at 37°C | Level (ng/mg) of intracellular accumulation | |
| 5 | 241 | 330 | 89 | 10 | 123 | 113 |
| 10 | 154 | 259 | 105 | 10 | 129 | 119 |