TABLE 3.
Nucleotide and predicted amino acid changes in HAs and NAs of viruses resistant to inhibitor BCX-140 (strains BCX-140RM, BCX-140RM2, and BCX-140R) compared to parental virus A/Singapore/1/57 passaged without inhibitor (strain C6A)
| Virus stock | NA changes
|
HA changesa
|
||
|---|---|---|---|---|
| Nucleotide | Amino acid | Nucleotide | Amino acid | |
| BCX-140RM (D6A) | C-484→T/C | G-483→A | Arg-132→Gln (HA1) | |
| T-964→C | ||||
| G-1061→G/C | Gly-348→Gly/Arg | |||
| A-1099→T | ||||
| BCX-140RM2 (D6A*) | T-964→C | G-485→A | Ala-133→Thr (HA1) | |
| G-630→C | Ser-204→Thr | |||
| G-951→G/C | Ser-311→Ser/Thr | |||
| BCX-140R | T-964→C | G-483→A | Arg-132→Gln (HA1) | |
a
The HA sequence contained the following changes compared to the HA sequence of A/Singapore/1/57 HA sequence in GenBank: G-539 to A (Glu-151 to Lys), A-639 to C (Lys-189 to Thr), C-877 to T, A-996 to T (Lys-303 to Ile), A-853 to A/T, A-1115 to G (HA Ile-218 to Val), T-1201 to C, G-1720 to A.