Fig. 4.

Influence of AKK and pAKK on the expression of tight junction proteins and RegIII lectins in Salmonella-infected mice. The mRNA expression of (A) Cldn3, (B) Ocln, (C) Tjp1, and (D) Muc2. (E) Western blots for tight junction proteins. (F) Quantification of bands in E. (G) Immunofluorescence image of tight junction proteins. The blue color represents colonic cells stained with DAPI, the red color represents Cldn3 and Ocln stained with Alexa Fluor  647, and the green color represents Tjp1 and Muc2 stained with Alexa Fluor  488. Images were taken at × 10 magnification. (H) The mRNA expression of Lcn2. (I) Lipocalin-2 levels determined by ELISA. The mRNA expression of (J) Reg3b and (K) Reg3g. (L) Western blots of RegIII proteins. (M) Quantification of bands in L. n = 3 ∼ 5. Statistical significance was determined by ANOVA for multiple comparisons and post-hoc Tukey test for comparisons between two groups. *p < 0.05, **p < 0.01, and ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)