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. 2023 Mar 28;52:89–102. doi: 10.1016/j.jare.2023.03.008

Fig. 6.

Fig. 6

Effect of AKK and pAKK on antimicrobial activity of macrophage and its NO and ROS production. The numbers of intracellular Salmonella in macrophages pretreated with (A) pAKK at different MOI for 24 h, (B) AKK at different MOI for 24 h, (C) pAKK or AKK at the same MOI for 24 h, and (D) pAKK or AKK at an MOI of 10 for 6 h or 24 h. (E) The antimicrobial kinetics of macrophages. (F) The fluorescence intensity of GFP-S. Typhimurium SL1314. (G) Representative image of GFP-S. Typhimurium. (H-N) Macrophages were pretreated with pAKK or AKK for 24 h, then infected with S. Typhimurium SL1314 for 3 h. After that, (H) ROS, (I) NO, (K) IL-1β, and (L) IL-6 were measured. (J) Western blotting (up) and band quantification (down) of iNOS. (M) Western blotting (left) and band quantification (right) of NLRP3. (N) Immunofluorescence image of NLRP3. (O) The number of intracellular Salmonella in macrophages pretreated with MCC950 for 24 h before pAKK treatment. Statistical significance was determined by ANOVA for multiple comparisons and post-hoc Tukey test for comparisons between two groups.*p < 0.05, **p < 0.01, and ***p < 0.001.