Fig. 1.

Effect of AMC water on diarrhea incidence and hypothalamus-pituitary-adrenocortical axis in MS piglets under weaning stress. (A) diarrhea incidence. (B-F) The serum levels of (B) CRH, (C) AVP, (D) ACTH, (E) COR and (F) Hpt. (G-J). Western blotting measurements of the protein levels of (H) POMC, (I) ACTH, and (J) CRHR1 in the pituitary gland. (K-O) Western blotting measurements of the protein levels of (L) GR, (M) MR, (N) BDNF, and (O) C-fos in the hypothalamus. (P) Heat map of mRNA levels of CRH, ARC, GR, MR, and FKBP5 in the hypothalamus, and CRHR1 and AVPR1B in the pituitary gland. Statistical analysis was performed using Student’s t-tests to compare differences between the two groups. Data are presented as the mean ± SD. ns, not significant, *P < 0.05, ^**P < 0.01, and ^***P < 0.001.