Fig. 3.

Effect of AMC water on marker bacterial, microbiota interaction network and functional profiles in MS piglet. (A) Linear discriminant analysis effect size (LEFse) analysis on gut microbiota. Linear discriminant analysis (LDA): an LDA score higher than 2 indicated a higher relative abundance in the corresponding group than in other groups. (B) LEfSe taxonomy cladogram: different colors suggest enrichment of certain taxa in AMC_15d (green), AMC_7d (red) and Con_7d (purple). The size of the circles is based on relative abundance. The significantly different species using the nonparametric factorial Kruskal–Wallis rank sum test at a significance level of 0.05. (C-D) Microbiota interaction network analysis of common core fecal bacteria in the (C) Con group and (D) AMC group. The red line means a positive correlation, and the green means negative correlation. (E) The comparative analysis for the relative abundances of bacterial KEGG pathways between the Con group and AMC group. P-value < 0.05 means a significant difference. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)