Fig. 4.

Effects of AMC water on interaction mediators of the brain-microbe axis in MS piglets under weaning. (A) Correlation analysis between HPA axis and gut microbes. (B) Fecal LPS levels. (C) Serum LPS levels. (D-G) The levels of proinflammatory factors (D) IL-1β, (E) IL-6, (F) TNF-α and (G) IFN-γ in the hypothalamus. (H-J) The mRNA fold expression of (H) IL-1β, (I) IL-6 and (J) TNF-α in the hypothalamus. (K) Correlation analysis between fecal/serum LPS, hypothalamus proinflammatory factors and brain-microbes axis. Statistical analysis was performed using Student’s t-tests to compare differences between the two groups. Data are presented as the mean ± SD. ns, not significant, *P < 0.05, ^**P < 0.01, and ^***P < 0.001.