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. 2022 Dec 17;52:29–43. doi: 10.1016/j.jare.2022.12.008

Fig. 6.

Fig. 6

Effect of AMC water on the number of goblet cells and Paneth cells in the small intestine of MS piglets. (A) The intestinal goblet cells shown by PAS staining of duodenum, jejunum, and ileum tissues in MS piglets (scale bar 100 μm). (B) Statistical analysis of the goblet cells number in the duodenum, jejunum, and ileum (n = 3). (C) The intestinal Paneth cells marked by Lys + of duodenum, jejunum, and ileum tissues in MS piglets. The mean optical density was analyzed by Image J software. (D) Statistical analysis of Lys + mean density in the duodenum, jejunum, and ileum (n = 3). (E) Heat map of mRNA levels of Mucins (Muc 1–6), lysozyme (Lyz1), and defensins (DEFB1-2) in duodenum, jejunum, and ileum tissues. The mRNA levels were determined by qRT-PCR (n = 6). Statistical analysis was performed using Student’s t-tests to compare differences between the two groups. Data are presented as the mean ± SD. ns, not significant, *P < 0.05, **P < 0.01, and ***P < 0.001.