Fig. 7.

Effect of AMC water on Wnt/β-catenin signaling pathway of small intestine in MS piglets. (A) Western blotting measurements of the protein levels of Wnt/β-catenin signaling pathway in small intestine (including duodenum, jejunum, and ileum) from MS piglets. (B) Western blotting measurement of the protein levels of nuclear β-catenin of duodenum, jejunum, and ileum in MS piglets. (C-J) The protein levels of (C) Wnt1, (D) total β-catenin, (E) Axin, (F) GSK-3β, (G) APC, (H) Lrp6, (I) Lrp5, and (J) nuclear β-catenin of duodenum, jejunum, and ileum in weaned-piglets. (K) Immunofluorescence images of β-catenin staining (red) and DAPI (blue) of duodenum, jejunum, and ileum in MS piglets. The mean density of β-catenin was detected. (L) Heat map of mRNA levels in Wnt/β-catenin signaling pathway. Statistical analysis was performed using Student’s t-tests to compare differences between the two groups. Data are presented as the mean ± SD. ns, not significant, *P < 0.05, ^**P < 0.01, and ^***P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)