Fig. 8.

Effect of AMC water on intestinal stem cells proliferation of small intestine in MS piglets. (A) Immunofluorescence images of Lgr5 + staining (red) and DAPI (blue) of duodenum, jejunum, and ileum in MS piglets. (B) Western blotting measurements of protein levels of cell proliferation markers of duodenum, jejunum, and ileum in MS piglets. (C) Mean density of Lgr5 + in duodenum, jejunum, and ileum of MS piglets. (D-F) The protein levels of (D) Lgr5, (E) Ki67, and (F) Cyclin D1 of duodenum, jejunum, and ileum in MS piglets. (G) Heat map of mRNA levels of cell proliferation markers (C-myc, CyclinD1, Ki67), active ISC markers (Lgr5, Olfm4, Ascl2), and quiescent ISC marker (Bmi1) in duodenum, jejunum, and ileum of MS piglets. Statistical analysis was performed using Student’s t-tests to compare differences between the two groups. Data are presented as the mean ± SD. ns, not significant, *P < 0.05, ^**P < 0.01, and ^***P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)