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. 2023 Sep 18;20(10):1573–1580. doi: 10.1038/s41592-023-02001-y

Extended Data Fig. 4. GelMap does not affect cellular morphology or macroscopic expansion.

Extended Data Fig. 4

a) U2OS cells grown on coverslips with increasing amount of (patterned) NBD: bare glass, coated coverslip completely illuminated with deep-UV, 40 µm grid size, 20 µm grid size, 10 µm grid size and completely coated. Representative examples from multiple fields of view in 2 biological replicates. b) Quantification of area and circularity of individual manually segmented cells for indicated conditions. N = 2 biological replicates, n = 110, 123, 127, 119, 114, 124 cells for: bare glass, coated coverslip completely illuminated with deep-UV, 40 µm grid size, 20 µm grid size, 10 µm grid size and completely coated, respectively. Data is represented as individual values with error bars indicating mean ± SD. c) Expanded gels produced using the indicated coated coverslips. d) Quantification of macroscopic expansion factor for indicated conditions. N = 3 replicates, error bars indicate mean ± SD. ns = not significant (Mann–Whitney U, two-tailed test). Scale bars: A: 100 µm, C: 50 µm.