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. 2023 Sep 18;20(10):1573–1580. doi: 10.1038/s41592-023-02001-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a) Representative images of expanded cells cultured on GelMap grids and stained for tubulin (cyan) and myc (orange) using the indicated Expansion Microscopy variant and conditions. Uncorrected images show the result of linear transformation of the expanded image to a virtual reference grid, while corrected images show the result of non-linear transformation. Representative zooms are shown together with the expansion factor measured using GelMap. b) Table summarizing the different anchoring, homogenization and staining/amplification strategies. Scale bars: all scale bars 100 µm, iterative pan-ExM 50 µm, all zooms 10 µm. Scale bars in expanded samples reflect pre-expansion sizes.