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. 2023 Oct 2;20(10):1530–1536. doi: 10.1038/s41592-023-02007-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, The scDVP workflow comprised embedding of fresh mouse liver tissue, staining and high-content microscopy, AI-guided hepatocyte segmentation, cutting and sorting of cells on a laser microdissection microscope, and peptide preparation with or without dimethyl labeling. The Δ0 channel contains the reference proteome and Δ4 and Δ8 contain two individual samples, which are all analyzed by ultra-high-sensitivity mass spectrometry. Created with BioRender.com. b, Liver painting with four stains. Left: E-cadherin marks PV regions, glutamate-ammonia ligase (Glul) surrounds the CV, the cell segmentation marker phalloidin, and the sinusoidal and nuclear counterstain WGA. Right: false color overlay of all channels: orange, E-cadherin; yellow, WGA, gray, phalloidin; turquoise, Glul. Scale bars, 100 μm.