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. 2023 Oct 5;220(12):e20231242. doi: 10.1084/jem.20231242

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© 2023 Chan et al.

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Figure 2. — Assessment of reporter specificity, image data processing, and TranslocQ compatibility. (A) Single-cell ERK-KTR versus RelA responses to αCD3/αCD28 versus TNFα stimulation added at the time point indicated by the dashed line. Time to activation (shown as population mean + s.d.) represents the time from adding stimulation to reporter translocation peaks. **P value <0.01, *P value <0.05, Mann–Whitney U test. ND, not determined. Results are representative of >3 independent experiments. (B and C) Population response curves (mean ± s.d.) versus single-cell AUC/AUP distributions (violin plots with median and quartiles) comparing the indicated CRISPR-KO or (C) chemical inhibitor conditions following αCD3/αCD28 antibody stimulation added at the indicated time point. ***P value between 0.0001 and 0.001, ****P value <0.0001, two-tailed unpaired t test. Results are representative of three independent experiments in B or two independent experiments in C.