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. 2023 Oct 5;220(12):e20231242. doi: 10.1084/jem.20231242

Figure 6.

Figure 6.

PD-L1:PD-1 engagement in the presence of CD80 expression inhibits both TCR55- and TCR55-CD28–dependent pathways. (A) Population response curves (mean ± s.d.) versus single-cell AUC distributions (violin plots with median and quartiles) comparing the indicated αPD-L1 antibody concentration titration from 0 to 1,000 ng/ml in the presence of PD-L1 expression following the addition of SQL-loaded (1 μM) APC at the indicated time point. Results are representative of two independent experiments. (B) Radar plots represent normalized mean values of corresponding single-cell AUC/AUP distributions comparing the indicated SQL peptide ligand quantity presented by CD80− (left) versus CD80+ (middle) versus CD80++ (right) APC in the absence or presence of PD-L1 expression as indicated. Results are representative of two independent experiments. (C) High CD80 expression and strong TCR signaling abolish PD-L1:PD-1 suppression. Radar plots represent normalized mean values of corresponding single-cell AUC/AUP distributions comparing the indicated Pep20 peptide ligand quantity presented by CD80++ APC in the absence or presence of PD-L1 expression as indicated. Results are representative of three technical replicates. Bar graphs show further normalization of these mean values within each comparison pair. ns: P value ≥0.05, *P value between 0.01 and 0.05, **P value between 0.001 and 0.01, ***P value between 0.0001 and 0.001, ****P value <0.0001, two-tailed unpaired t test.