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. 2023 Oct 5;220(12):e20231242. doi: 10.1084/jem.20231242

Figure 9.

Figure 9.

PD-L1:PD-1 engagement inhibits TCR6 signaling efficiently in the absence but not in the presence of CD80 expression. (A) Radar plots represent normalized mean values of corresponding single-cell AUC distributions in the absence or presence of PD-L1 blocking antibody as indicated. Bar graphs show further normalization of these mean values within each comparison pair. (B) PCA of population response dynamics comparing the indicated peptide ligand quality versus quantity, in the absence versus the presence of PD-L1 blocking antibody. Top: The loadings of signaling responses on PC1 (+0.61*ERK+0.67*NFAT2+0.43*p38). Bottom: The projection of signaling responses plotted along the PC1. (C) Radar plots represent normalized mean values of corresponding single-cell AUC/AUP distributions in the absence or presence of PD-L1 blocking antibody. Bar graphs show further normalization of these mean values within each comparison pair. ns: P value ≥0.05, **P value between 0.001 and 0.01, ***P value between 0.0001 and 0.001, ****P value <0.0001, two-tailed unpaired t test. Results are representative of two independent experiments.