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. 2023 Oct 5;220(12):e20231242. doi: 10.1084/jem.20231242

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© 2023 Chan et al.

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Figure S2. — CD3, CD28, and PD-1 expression and signaling profiles induced by TCR55 versus TCR55+CD28 receptor engagement. (A) CD3 and CD28 expression profiles following CRISPR/Cas9-mediated KO and MACS-based purification. Reporter Jurkat cell lines (ERK-RelA, JNK-NFAT2, or p38) versus genotypes (wild-type control, CD3E KO, or CD28 KO) are indicated. (B and D) Radar plots represent normalized mean values of corresponding single-cell AUC/AUP distributions in the (B) absence or (D) presence of PD-1 expression as indicated and shown in Fig. 4 cartoons. Results are representative of two independent experiments. (C) Jurkat reporter PD-1 expression profiles following lentiviral transduction of the LE-hPDCD1 vector and MACS-based purification.