Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 5;14:6214. doi: 10.1038/s41467-023-41999-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Left panels, representative confocal images of mouse colonoid monolayers expressing CLDN23 at the plasma membrane. Nuclei stained with DAPI (blue) Middle panels show positive PLA signal (magenta dots) at the cell-cell contact (arrow) between CLDN23 with either CLDN3 or CLDN4 in mouse colonoids. Right panels, show merged images. Scale bars: 20 μm. b, c Left panel, interaction energy landscapes obtained from PANEL method for b homomeric and c heteromeric interactions of CLDN3 and CLDN4 with CLDN23 in which the known pore-forming rotational orientation was indicated at 270° ± 10 for each CLDN (green arrow). Right panel, representative in silico ribbon diagrams of pore-forming homomeric (b) and heteromeric (c) interactions of CLDN3 (light purple), CLDN4 (light purple), and CLDN23 (gray). d Bar plot showing a comparison of energy values of pore-forming homodimers and heterodimers of CLDN3, CLDN4, and CLDN23. Results show the mean ± SD of 400 data points corresponding to the known pore-forming rotational orientation on the landscape. ****p ≤ 0.0001; one-way ANOVA with Tukey’s posttest. e Bar plot showing energy values of non-pore-forming rotational orientations. Results show the mean ± SD of 12,960 data points. ****p ≤ 0.0001; one-way ANOVA with Tukey’s posttest.