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. 2023 Oct 5;10:678. doi: 10.1038/s41597-023-02595-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Characteristics of identified isoforms. Plots showing the length of isoforms (a), the number of exons (b), the length of coding sequence (c), the length of ORF (d), the proportion of protein-coding genes(e), and the proportion of predicted NMD (f) in known isoforms (FSM, ISM) and novel isoforms (NIC, NNC, Genic, Antisense, Fusion, Intergenic). Histogram showing the distance to annotated transcription start site (TSS) (g) and transcription termination site (TTS) (h) for FSM and ISM. (i) Number of isoforms per gene. (j) Single-nucleotide polymorphism (SNP) count using 100 kb of sequencing length as window size in the ten samples. Note: NMD, nonsense-mediated mRNA decay; FSM, full splice match; ISM, incomplete splice match; NIC, novel in catalog; NNC, novel not in catalog. Tes, testis; Epi, epididymis; VG, vesicular gland; PG, prostate gland; BG, bulbourethral gland.