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. 2023 Oct 5;10:678. doi: 10.1038/s41597-023-02595-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Analysis of novel coding isoforms and novel non-coding isoforms. (a) Percentage of isoforms in each annotation category. (b) Number of coding isoforms predicted by four approaches: CPC2, CPAT, GMST and Pfam. The pink bar indicates the union of predicted coding isoform from four approaches. (c) The number of lncRNA in each category. (d) The scatter plot shows the number of exons (x axis) and length distribution (y axis) of novel lncRNA, known lncRNA and known mRNA. Green dot: novel lncRNA; Orange dot: known lncRNA; Blue-gray: known mRNA. The vertical dashed line represents the 5 exons. The horizontal dashed line represents the 1 kb transcript length (e) Sequence annotation analysis for novel coding isoforms. The red points represent significance (log10(e-value) <−5) according to both NR (Non-Redundant protein sequence database) and Uniprot (Universal protein database). Blue points indicate NR only, purple points indicate UniProt only, and green points indicate no significance in either analysis.