Fig. 2.

Structure of different CAR-T generations. Compared to the first generation, that contained only one intracellular component, CD3ζ, the second- and third-generation CARs include one or two co-stimulatory domains, respectively. The fourth generation of CARs is based on second generation with the addition of an inducibly expressed chemokine. The fifth generation is characterized by the incorporation of a truncated cytoplasmic domain of IL-2Rβ for STAT5 recruitment and a STAT3-binding YXXQ motif allowing activation of the JAK/STAT signaling after antigen engagement