Figure 1.

Overview of single-cell transcriptome profiling conducted in a murine model of acute kidney injury and MSC therapy

(A) Summary of the IRI-AKI model, single-cell RNA sequencing, and functional experiments. (B) Biochemical detection of representative renal function indicators (Scr, serum creatinine; BUN, blood urea nitrogen) in IRI-AKI- and MSC-treated kidneys. (C) Acute tubular necrosis (ATN) scores. (D) PAS staining, H&E staining, and immunofluorescence staining of CD45 in mouse IRI-AKI- and MSC-treated kidneys. (E and F) Distribution of 102,162 high-quality kidney cells by cell type (E) and treatment group (F). (G) Heatmap showing the expression level of top differentially expressed genes (DEGs) in each cell type. (H) Violin plot showing the expression of canonical markers in each cell type. (I) Proportions of each cell type in distinct sample groups. Data are expressed as mean ± SD (n = 8); one-way ANOVA was used for comparisons of three or more groups. ∗∗p < 0.01.