Figure 3.

MSC treatment promoted tissue repair by enhancing renal stem/progenitor epithelium differentiation

(A) Potential renal stem/progenitor epithelial cell differentiation routes revealed by trajectory analysis. (B) Heatmap showing the scaled expression of dynamic genes along the two branches of the renal stem/progenitor epithelial trajectory. (C) Trajectory analysis showing the dynamic expression of representative stem cell and fibrosis genes. (D) Enriched GO terms for modules 1–4. (E) mRNA levels of Pcna, and Prom1 (Cd133) in sham-, IRI-AKI-, and MSC-treated kidneys. (F) Western blotting and semi-quantitative analysis of PCNA and CD133 protein levels in sham-, IRI-AKI-, and MSC-treated kidneys. Data are expressed as mean ± SD (n = 4); one-way ANOVA was used for comparisons of three or more groups. ∗p < 0.05, ∗∗p < 0.01.