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. Author manuscript; available in PMC: 2023 Oct 23.
Published in final edited form as: Cell Rep. 2023 Apr 6;42(4):112335. doi: 10.1016/j.celrep.2023.112335

Figure 1. Human neurons metabolize glucose through glycolysis.

Figure 1.

(A and B) (A) Cellular and (B) media-derived metabolites from neurons expressing either an NTG guide (control) or a CRISPRi sgRNA targeting pyruvate kinase (PKM KD). Groups were compared for fractional [U-13C]glucose labeling (top) and total relative metabolite levels (bottom). See Figure S1D for corresponding isotopologue data. Data are means ± SEM. n = 4 samples/group.

(C) Schematic of PKM KD impact on [U-13C] glucose metabolism.

*p < 0.05, **p < 0.01, and ***p < 0.001 by Welch’s t tests.