| Condition | Metabolic probe | Description |
|---|---|---|
| 1 | [U-13C]glucose 20.5 mM | Differentiation media with basal level of glucose |
| 2 | [U-13C]glucose 1.5 mM | Physiologically relevant brain glucose level |
| 3 | [U-13C]glucose 1.5 mM | 1:1 glucose:galactose media tracing glucose |
| 4 | [U-13C]galactose 1.5 mM | Galactose replacing all glucose from condition 2 |
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