Figure 4.

LNP cell-type expression in the brain

Mice received bilateral intracerebral injections in the prefrontal cortex with cre-mRNA-LNPs that express tdTomato (red) following Cre-recombination. (A) High (top) and low (bottom) magnification of tdTomato florescence and injection placements in the prefrontal cortex. Scale bars, 200 μm. (B) Quantification of tdTomato+ cells preferentially co-expressed with neuronal (NeuN+, blue) over microglial (Iba1+, yellow) or astrocyte (GFAP+, green) markers (nuclear stain, DAPI, blue). (C) TdTomato+ cells overlap slightly with Iba1+ cells (green arrows), but mostly co-express with NeuN+ cells (white arrows). (D) TdTomato+ cells do not co-label with GFAP+ cells. Scale bars, 25 μm. n = 4, mean ± SEM.