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. 2022 Jul 29;11(1):36–44. doi: 10.2174/2211536611666220218121640

Fig. (4).

Fig. (4)

MiR-206 overexpression regulates ERα, p68 RNA helicase, and HMGA2. A) On the left, it shows how the overexpression of the pSuper.neo.GFP-pre-miR-206 (white columns) decreases cell growth in HeLa cells, (*) P=0.01 and (**) P=0.05 (Student’s t-test). On the right, the table shows the Taqman quantification of the overexpression of mature miR-206. B) Western blots, incubated with anti-ERα, anti-p68 RNA helicase, anti-HMGA2, and anti-GAPDH, with protein extracts from wild-type HeLa cells transiently co-transfected with pSuper.neo.GFP-pre-miR-206 vector (lane 1) and pSuper.neo.GFP empty vector (lane 2). C) Wild-type HeLa cells transiently transfected with psiCHECK-HMGA2 reporter vector containing the HMGA2 3’-UTR. Lane 1: wild-type HeLa cells transiently transfected with pSuper.neo.GFP; and lane 2: with pSuper.neo.GFP-pre-miR-206 vector. MiR-206 action, normalized by the Rluc/Fluc detection assay, shows a 1.7-fold decrease in HMGA2 3’-UTR (in column 2, P<0.01, Student’s t-test). Three independent experiments were performed, each of them per triplicate.