Table 1.
Summary of immunohistochemical methods: primary antibody details, source, dilution, and blocking solution.
| Specificity/Clone | Type of antibody | Source | Blocking solution | Dilution |
|---|---|---|---|---|
| IBA1 | mAb (clone, GT10312) | Invitrogen, MA, United States | Superblock1 | 1:1002 |
| MAC387 | pAb | Bio-techne, OX, United Kingdom | 10% NGS | 1:1002 |
| MPO | pAb | Invitrogen, MA, United States | Superblock1 | 1:1002 |
| CD3 | mAb (clone, LN10) | Leica Microsystems, MK, United Kingdom | Superblock1 | 1:503 |
| B-Cell-SAP | mAb (clone, DAK-Pax5) | Agilent, CA, United States | Superblock1 | 1:503 |
1
Superblock (TBS) Blocking Buffer (Thermo Scientific, United States).
2
Primary antibody incubation 1 h in the oven at 37°C.
3
Primary antibody incubation overnight at 4°C.
MAb, monoclonal antibody; pAb, polyclonal antibody; NGS, normal goat serum; MPO, myeloperoxidase; B-cell SAP, B-cell Specific Activator Protein.