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. 2023 Sep 22;14:1261180. doi: 10.3389/fpls.2023.1261180

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Copyright © 2023 Sanchez Carrillo, Hoffmann, Barff, Beck and Germain

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protoplasts vitrification by plunge-freezing and Correlative Light and Electron Microscopy (Steps 19 - 29 and 40 - 47) (A), Diagram showing the steps to follow after protoplast isolation: Plunge freezing procedure and observing the EM grids containing the root protoplasts under a cryo-CLEM, (B-D), Cryo-Fluorescence Microscopy of a grid containing root-protoplasts from Arabidopsis thaliana’s RAE1-GFP transgenic line. Brightfield: field of bright light; GFP: Green fluorescence signal (green); Merge shows the overlay of the brightfield and GFP signal.