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. 2023 Sep 25;120(40):e2300489120. doi: 10.1073/pnas.2300489120

Fig. 3.

Fig. 3.

Nuclear TIAM1 promotes NSCLC cell migration dependent on RAC1. (A) Representative images of H441 cells migrating through a wound shaded as in 2A, transfected with TIAM1 or control siRNAs and inducibly expressing NLS-TIAM1 (resistant to siTIAM1#2), following the addition of doxycycline. (Scale bar, 200 μm.) The graph shows percentage wound closure relative to initial wound size. (B) Representative images of H441 cells migrating through Transwell inserts, transfected with TIAM1 or control siRNAs and inducibly expressing NLS-TIAM1 following the addition of doxycycline. (Scale bar, 1,000 μm.) The graph shows average percentage of migrating cells where control cell migration is set to 100%. (C) Representative images of H441 cells migrating through Transwell inserts, transfected with TIAM1 or control siRNAs and inducibly expressing NLS-TIAM1 following the addition of doxycycline. Cells were treated with 20 μM NSC23766 (+) or DMSO control (−). (Scale bar, 1,000 μm.) The graph shows the average percentage of migrating cells relative to siControl cells, normalized to cell viability. Data are presented as mean ± SEM of three independent experiments. ns = nonsignificant, *P < 0.05, **P < 0.01, and ***P < 0.001 (ANOVA).