Fig. 7.

Protective role of IL-27RA during pulmonary fibrosis. (A) Lung sections of IL-27RA^−/− mice and WT mice during bleomycin-induced pulmonary fibrosis, Masson–Goldner’s trichrome staining, n ≥ 6/group (Scale bar, 50 µm). (B) Quantification of collagen deposition using scanned whole slides from mice in frame A. (C) Expression of collagen I mRNA in lung tissue of IL-27RA^−/− and WT mice after bleomycin, RT-PCR. (D) SIRCOL protein assay for collagen I–V in lung tissue of the experiment described under C. (E) Flow cytometry of CD4⁺ T cells in BALF of bleomycin i.t.-treated IL-27RA^−/− mice as compared to WT mice. (F) Collagen accumulation after bleomycin-induced pulmonary fibrosis in IL-17A^−/−IL-17F^−/− double-knockout mice as compared to WT mice, SIRCOL assay. All frames were analyzed at 4 wk after bleomycin and frames B–F were done with numbers of mice as indicated by circles; Student’s t test or one-way ANOVA; *P < 0.05, **P < 0.01.