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. 2023 Sep 26;9(10):e20428. doi: 10.1016/j.heliyon.2023.e20428

Table 10.

The antibacterial activity of chalcones.

Compound(s) “Chemical name(s)” Bacterial species Study model(s) Results Inhibition mechanism Refs.
Thiazole-based chalcones “(E)-3-(2-chloro-6-fluorophenylo-1-(2-(ethylamino)- 4–2.1.4.methylthiazol-5-yl)prop-2-en-1-on” Pseudomonas aeruginosa, MRSA, and Escherichia coli In vitro,
In silico
MIC: 0.65, 1, 1.3 μmol/mL × 10−2 against E. coli, P. aeruginosa, and MRSA, respectively Inhibition of DNA gyrase, GyrB, and MurA [125]
“3-(4-Trifluoromethylphenyl)-1-(2-hydroxyphenyl)-2-propen-1-on”
“3-(2,6-dimethoxyphenyl)-1-(2-hydroxyphenyl)-2-propen-1-on”
“3-(2-methoxyphenyl)-1-(2-hydroxyphenyl)-2-propen-1-on”
“3-(4-fluoro-2-methylphenyl)-1-(2-hydroxyphenyl)-2-propen-1-on”
P. aeruginosa and Acinetobacter baumannii In vitro MICs: 100–175 μg/mL Supported by methoxy and halogen groups [126]
2′-Hydroxychalcones “(E)-3-(2-hydroxyphenyl)-1-phenylprop-2-en-1-one” MRSA strains including RN4220, K4414, 1199B, and K2068 In vitro,
In silico
Binding energies: 6.4, −7.4,
−7.0, −7.2, −7.5, and −7.2 kcal/mol
Inhibition of the NorA efflux pump [130]
Azidosulfonamide chalcones “N-[4-[3-(4-Bromophenyl)acryloyl]phenyl]-4-azidobenzene- sulfonamide” S. aureus, Micrococcus luteus, Serratia marcescens, Klebsiella pneumoniae, and E. coli In vitro,
In silico
IZDs: 33 mm and MIC: 1.5 μg/cm3 against S. aureus;
IZD: 29 mm and MIC: 1.5 μg/cm3 against M. luteus (most active compound)
Possible inhibition of the microbial DHPS enzyme [132]
Pyrazole-based adamantyl chalcones
“2-(3-((1S,3S)-adamatan-1-yl)-1-(2,4-dinitrophenyl)-4,5-dihydro-1H-pyrazol-5-yl)pyridine”
P. aeruginosa, E. coli, S. aureus, K. pneumonia, Salmonella typhimurium, and Bacillus subtilis In vitro IZDs: 15.3 mm against K. pneumonia (most active compound) N/A [37]
“(E)-3-(4-fluorophenyl)-1-(2-hydroxyphenyl)prop-2en-1-one”
“(E)-1-(2-hydroxyphenyl)-3-(4-ethoxyphenyl)prop-2en-1-one”
“(E)-3-(4-(dimethylamino)phenyl)-1-(2-hydroxyphenyl)prop-2-en1-one” “(E)-1-(2-hydroxyphenyl)-3-(thiophen-2-yl)prop-2-en-1-one”
E. coli ATCC 25922, S. aureus ATCC 25923, and the S. aureus 11999-B strain overexpressing the norA gene In vitro,
In silico
Significantly reduced the MICs of norfloxacin from 64 to 8 μg/mL Reduced the resistance to norfloxacin by inhibiting the NorA reflux [134]
4′-Hydroxy-3-4-dimethoxy-chalcone, 3′-hydroxy-3-acetate, 4-methoxy-chalcone, 3′,4′-dihydroxy, 3,4,4′-trimethoxy-chalcone, and 3,4-dimethoxy-chalcone SA1199B strain In vitro Strengthened the effects of norfloxacin and EtBr against the SA1199-B (norA) strain Competes with norfloxacin for similar MepA and NorA binding sites [135]
Thiazolyl chalcones
“3-(5-[4,5-Dihydro-5-(1H-pyrrol-2-yl)-1H-pyrazol3-yl]-4-methylthiazol-2-yl)pyridine”
“4-(4,5-Dihydro-3-[4-methyl-2-(pyridin-3-yl)thiazol-5-yl]-1H-pyrazol-5-yl)pyridine”
B. subtilis, Shigella flexneri In vitro MIC: 0.48 μg/Ml (most active compound) Results indicate that the effect of pyrazolines derivatives on Gram negative bacterial activity is attributed to its NH group which can act as a hydrogen donor to the target receptor [136]
Trifluoromethyl- and trifluoromethoxy-substituted chalcones “(E)-3-(1”H-indol-3″-yl)-1-[40 -(trifluoromethoxy)phenyl]prop-2-en-1-one”
“(E)-3-(1”H-indol-3″-yl)-1-[40 -(trifluoromethyl)phenyl]prop-2-en-1-one”
S. aureus, B. subtilis, E. coli, and, Proteus vulgaris In vitro MIC: 24 μM against B. subtilis and E. coli, MIC: 25 μM against E. coli and P. vulgaris, respectively N/A [137]
Fluorinated chalcones “(E)-3-(2′-ethoxyphenyl)-1-(4-Fluoro-2-hydroxyphenyl) prop-2-en-1-one”
“(E)-3-(3′,4′-diethoxyphenyl)-1-(4-fuoro-2-hydroxyl-ph enyl)prop-2-en-1-one” “(E)-3-(3′,5′-Bis[trifuoromethyl]phenyl)-1-(4-Fluoro- 2-hydroxylphenyl)prop-2-en-1-one”
MRSA, S. aureus, E. coli, S. pyrogenes, Vancomycin-resistant enterococci, Enterococcus faecalis, Salmonella typhi, Proteus mirabilis, Pseudomonas aeruginosa, Corynebacterium ulcerans In vitro IZD: 28 mm against E. coli; 28 mm against MRSA; 28 mm against S. pyrogenes The electron-releasing group on the B-ring and the fluorine atom on the A-ring enable easy penetration into the negatively charged bacterial wall. [138]

IZD: Inhibition zone diameter, MRSA: Methicillin-resistant Staphylococcus aureus, MIC: Minimal inhibitory concentration.