Table 2.
Genes isolated from tea plants in amino acids transport.
| Gene name | Sequence information | Functional verification | Reference | ||||
|---|---|---|---|---|---|---|---|
| Gene ID a | Cultivar b | Subcellular localization | Substrate c | System d | Function description | ||
| CsAAP1 | TEA031577.1 | SCZ | Plasma membrane and endoplasmic reticulum | Thea, Val, Asp, Glu, Gln, Ala, GABA | Yeast; in planta (Nt. At.) |
Highly correlated to Thea root- to- shoot transport | (Dong et al., 2020; Li et al., 2020) |
| CsAAP2 | TEA009392.1 | SCZ | Plasma membrane and endoplasmic reticulum | Thea, Val, Asp, Glu, Gln, Ala, GABA | Yeast; in planta (Nt. At.) |
– | (Dong et al., 2020; Li et al., 2020) |
| CsAAP3 | TEA003112.1 MK532959 | SCZ; LJ43 | Predicted:plasma membrane | – | – | – | (Guo et al., 2020) |
| CsAAP4 | TEA030129.1 MK532960 | SCZ; LJ43 | Predicted:plasma membrane | Thea, Val, Asp, Glu, Gln, Ala, GABA | Yeast | – | (Dong et al., 2020; Guo et al., 2020) |
| CsAAP5 | TEA033139.1 | SCZ | – | Thea, Val, Asp, Glu, Gln, Ala, GABA | Yeast | – | (Dong et al., 2020) |
| CsAAP6 | TEA013446.1 MK532961 | SCZ; LJ43 | Plasma membrane and endoplasmic reticulum | Thea, Val, Asp, Glu, Gln, Ala, GABA | Yeast; in planta (Nt. At.) |
– | (Dong et al., 2020; Guo et al., 2020; Li et al., 2020) |
| CsAAP7 | TEA005296.1 MK532962 | SCZ; LJ43 | Predicted:plasma membrane | – | – | – | (Guo et al., 2020) |
| CsAAP7.1 | XM_028244216.1 | SCZ | – | – | – | – | (Li et al., 2022) |
| CsAAP7.2 | MG523885 | SCZ | Endoplasmic reticulum | Thea, Ala, GABA, Ser, Glu, Asn, Pro | Plays a role in AAs uptake from soil and Thea long- distance transport | (Li et al., 2022) | |
| CsAAP8 | TEA031424.1 MK532963 | SCZ; LJ43 | Predicted:plasma membrane | Thea, Val, Asp, Glu, Gln, Ala, GABA | Yeast | – | (Dong et al., 2020; Guo et al., 2020) |
| CsAAP9 | TEA000756.1 | SCZ | – | – | (Dong et al., 2020) | ||
| CsLHT1 | TEA026462.1 | SCZ; LJ43 | Plasma membrane | Glu, Gln, Ala, Pro, Asn, Asp, GABA | H+-dependent high affinity transporter in uptake AAs from soil | (Guo et al., 2019; Li et al., 2021) | |
| CsLHT2 | TEA021847.1 | SCZ | Predicted:plasma membrane | – | – | – | (Li et al., 2021) |
| CsLHT3 | TEA033469.1 | SCZ | Predicted:plasma membrane | – | – | – | (Li et al., 2021) |
| CsLHT4 | TEA029168.1 CSS0010852.1 |
SCZ; FD | Predicted:plasma membrane | – | in planta (At.) |
– | (Li et al., 2021; Huang et al., 2023b) |
| CsLHT5 | TEA016092.1 | SCZ | Predicted:plasma membrane | – | – | – | (Li et al., 2021) |
| CsLHT6 | TEA003706.1 | SCZ; LJ43 | Plasma membrane | Glu, Gln, Ala, Pro, Asn, Asp, GABA | H+-dependent low affinity transporter in uptake AAs from soil | (Guo et al., 2019; Li et al., 2021) | |
| CsLHT7 | TEA021821.1 CSS0033052.1 |
SCZ; FD | Predicted:plasma membrane | – | in planta (At.) |
– | (Feng et al., 2018; Li et al., 2021) |
| CsLHT11 | CSS0019144.1 | FD | – | in planta (At.) |
(Huang et al. 2023b) | ||
| CsLHT8.1 | – | LJ43 | Predicted:plasma membrane | – | – | – | (Guo et al., 2019) |
| CsLHT8.2 | – | LJ43 | Predicted:plasma membrane | – | – | – | (Guo et al., 2019) |
| CsCAT1 | KY709681 | SCZ | – | – | – | – | (Feng, 2017; Feng et al., 2018) |
| CsCAT2 | KY709679 | SCZ | Tonoplast | Thea, Asp, Glu, Ala, Gln, Val | Meditate Thea storage | (Feng, 2017; Feng et al., 2018; Feng et al., 2021) | |
| CsCAT5 | KY709680 | SCZ | – | – | – | – | (Feng, 2017; Feng et al., 2018) |
| CsCAT6 | KY709682 | SCZ | – | – | – | – | (Feng, 2017; Feng et al., 2018) |
| CsCAT8 | KY709684 | SCZ | – | Thea, Glu, Gln, | – | – | (Feng, 2017; Feng et al., 2018) |
| CsCAT9 | KY709683 | SCZ | – | Thea, Glu, Gln | – | – | (Feng, 2017; Feng et al., 2018) |
a
Gene ID, the beginning as “TEA” and “CSS” can be found in the tea plant genome database (http://tpia.teaplants.cn), others are GenBank accession numbers (https://www.ncbi.nlm.nih.gov/genbank/);
b
Cultivar, SCZ, ShuChaZao; LJ43, LongJing43; FD, FudingDaBaiCha.
c
Substrate, Thea, theanine; Glu, glutamate; Gln, glutamine; Asp, aspartate; Asn, Asparagine; Ala, alanine; Val, valine; Pro, proline; Ser, serine; GABA, γ-aminobutyric acid.
d
System, At, Arabidopsis thaliana; Nt, Nicotiana tabacum.
-, related information not presented or studied in corresponding literature.