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. 2023 Oct 5;6(12):e202302148. doi: 10.26508/lsa.202302148

Figure 3. Activation of HIF-1ɑ impinges upon gene regulation of glucose metabolism.

Figure 3.

(A) Immunofluorescence showed HIF-1ɑ protein localization and expression in monocyte-derived macrophages (MDMs). Blue: DAPI; green: HIF-1ɑ. Scale bar, 300 μm. (B, C) MDMs were pretreated with or without LW6 (15 μM) for 2 h, followed by HIV-1 infection for another 48 h. (B, C) RT–qPCR analysis showed the mRNA levels of key genes in the glycolysis (B) and TCA cycle (C). (Statistical analysis was performed using a t test, *P < 0.05, **P < 0.01, and ***P < 0.001.) (D, E) MDMs treated with or without YC-1 (1 μM) and infected with or without HIV-1 were assessed for expressions of key genes in the glycolysis (D) and TCA cycle (E). (Statistical analysis was performed using a t test, *P < 0.05, **P < 0.01, and ***P < 0.001).