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. 2023 Oct 5;6(12):e202302148. doi: 10.26508/lsa.202302148

Figure 4. Effect of HIF-1ɑ inhibition on glycolysis, mitochondrial activity, and M1 polarization in monocyte-derived macrophages (MDMs).

Figure 4.

MDMs were infected with HIV-1 for 48 h, in the presence or absence of LW6 (15 μM) pretreatment (2 h). (A) Lactate concentration in the culture supernatant of MDMs. (Statistical analysis was performed using a t test, **P < 0.01 and ***P < 0.001.) (B) Mitochondrial membrane potential was assessed by the JC-1 assay. (Statistical analysis was performed using a t test, *P < 0.05.) Scale bar, 100 μm. (C, D) mRNA (C) and protein levels (D) of TNF-α, IL-1β, and IL-6 were detected using RT–qPCR and ELISA, respectively. (Statistical analysis was performed using a t test, *P < 0.05, **P < 0.01, and ***P < 0.001).